BIOR25 Molecular Ecology and Evolution 15 ECTS credits

Course description

Molecular DNA techniques are now instrumental in a wide range of applications from conservation biology to evolutionary ecology. You will learn how to use such methods in the lab and in computer based analyses and to read scientific papers using molecular techniques. It is a practically oriented course providing the basics for DNA analyses in any kind of free living organism. Steps included are:

  • DNA extraction
  • PCR
  • DNA sequencing
  • primer design
  • microsatellite analyses
  • phylogenetic reconstructions
  • population genetic analyses of DNA sequences and polyallelic markers

Computer programs that will be used include:

  • BioEdit
  • MEGA
  • DNAsp
  • FSTAT
  • STRUCTURE

To provide a common basis for the course, we start with basic lectures and a two-day excursion. The course is organized in five blocks. The first block is about using DNA sequencing for species identification (barcoding) and phylogenies. We then continue with analyses of genetic variation within species for genetic identification of individuals, parents and populations. The third block brings up analyses of coding genes for specific traits and how to use candidate genes to find the genetics behind adaptations. We then move from single genes to genomes, introducing the microarray technique, bioinformatics and how to make use of information in large genomic databases. At the end of the course you will carry out a ten day project of your own in one of the research groups in Ecology.

Exam

There is a written exam before the start of the individual projects, and to pass the course you also have to take part in the wet and dry labs during the course period. The course will end with oral presentations of the individual projects.

Aims

For students in ecology and evolution who want to learn the basics of molecular techniques to study wild species of animals, plants, fungi, bacteria, etc. For students in molecular biology who want to learn how molecular techniques can be applied to ecological and evolutionary questions in non-model organisms.


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Page manager: Inger Ekström
Questions about the website: Web Group
Publisher: Department of Biology

Last modified 1 Feb 2013

Study coordinator

Lotta Persmark
Student Advisor
Education office

Phone:
+46462223728

E-mail:
lotta.persmark@biol.lu.se

Course coordinators

Staffan Bensch
Professor
MEMEG

Phone:
+46462224292

E-mail:
staffan.bensch@biol.lu.se

Staffan Bensch
Professor
MEMEG

Course literature
Course period

The course will be given during the first part of the spring semester.

Lund University, Box 117, SE-221 00 Lund, Sweden. Tel: +46 (0)46 222 00 00